First, a bit of context. I’m an experimental biologist, not a computational biologist (at least, not primarily computational), so I have to deal with the fact that not everything in my research can be “electronified.” I’ve got samples in the freezer, vials of things in the fridge, cultures going in the incubators, and so on. Thus, I’m not looking for a notebook where I can keep every single bit of my research; maybe when the Matrix Google finally digitizes all of reality, I can finally just plug into my computer and never leave my desk.

On the other hand, though I’m not a computational biologist, I am pretty comfortable with computers. I program, I know HTML, and I can use Photoshop pretty quickly. I’m fine with cobbling together my own electronic lab notebook of sorts from the tools I can find on the internet (such as making a wiki); others (such as some in my lab) might find even formatting a wiki post to be an intimidating prospect. So what I do might not work for you.

Right now, my lab has an internal wiki using the MediaWiki engine, and I’ve been using my wiki space as my lab notebook. The wiki is backed up regularly, is password-protected, lets me view and edit the wiki from any internet-connected computer, has full-text search built-in, and has an editing format lets me put in cross-references to other entries; this is all I generally need for a lab notebook.

How do I manage the offline stuff, like gel pictures, data sheets, and so on? Well, I can put a surprising number of things on the wiki (for example, the lab next door lets me use their UV transilluminator, which has a CCD camera that I use to save TIFF images of my gels onto my network account), but basically I keep everything in a giant binder, numbered in order by date, and then put a reference to it in the online notebook. That way, I can easily find a result; just search the wiki for the entry I’m looking for, look up the reference number, and then find it in my binder of results. I do something similar with my experimental samples; my initials, followed by an experiment number, and then a vial number (such as “EJS-109-10″).

Unlike some people, my note-taking philosophy is that the lab notebook shouldn’t necessarily be a dirty log of absolutely everything that I do or think about while I’m in the lab; I don’t put in routine calculations or procedures, such as cell culture maintenance, making common reagents, and so on. The whole point of a lab notebook is so that I can keep track of what I did, so if I, or someone else, needs to look up what I did, or what’s in a vial in the freezer, I don’t have to spend hours trying to remember what I meant with “RfMQ2-3a-4-5-07″ on a tiny tube cap.

Sure, it’s sometimes nice to have everything in one lab notebook, gels pasted in and so on, but frankly, I find that even just the organizational benefits of being able to read my own handwriting and being able to search and cross-reference my posts to be worth giving up the all-in-one solution. When I kept a paper notebook, I spent so much time flipping back and forth between pages trying to remember where I’d written the concentration for the vial in my hand. Now, I just search for the vial number and voila!

Not only that, but I like to organize my lab notebook by project, rather than chronologically, because I generally have more than one thing going on at the same time. Organizing that way is doable on paper if you use a binder and loose-leaf paper, but still quite a hassle, especially since some experiments don’t always fit cleanly in one project or another, making it hard to find later (“Did I file it under this project or the other one?”). I prefer the electronic notebook, which lets me organize by both time and project, and lets me put experiments under more than one lab notebook by simply putting a link to it from both project pages. Not only that, but if I’m repeating an experiment with slightly different conditions, I can simply copy-and-paste a previous experiment and change just a few things.

Paper just doesn’t cut it for me anymore. If I were keeping a paper notebook, I’d basically be doing all the same things as I do on my electronic notebook: a loose-leaf notebook organized by projects, numbered experiments, separate binder of raw data, cross-referencing based on page numbers or experiment numbers, and so on. My online notebook does all this and adds remote access, readability, copy-and-paste, and searching to boot.

Not only that, but because my lab notebook is online, if I’m writing a paper or making slides for a presentation, I can be lazy and work from home. Win for the web!